Application of Bacilli Phages for Controlling Bacillus Subtilis Associated Date Palm Plantlets Malformation in Tissue Culture

M., Lewaa L.; A., Othman B.; M., Metwaly A.; A., El Dougdoug K.

doi:10.21608/esjp.2023.370487

Application of Bacilli Phages for Controlling Bacillus Subtilis Associated Date Palm Plantlets Malformation in Tissue Culture

Document Type : Scientific articles

Authors

¹ The Central Laboratory of Date Palm Researches and Development, Agricultural Research Center, Giza, 12619, Egypt

² Agricultural microbiology Dept. Faculty of Agriculture- Ain Shams University, Egypt.

³ The Central Laboratory of Date Palm Researches and Development, Agricultural Research Center, Giza, 12619, Egypt.

10.21608/esjp.2023.370487

Abstract

Date palm considered one of the most important crops in Arab region. Bacillus subtilis causes malformation on date palm in tissue culture stage. Three bacteriophages were isolated specific to Bacillus subtilis thus named BS1, BS2, and BS3. Results reveled that morphology of isolated phages belonged to family Myoviridae, Siphoviridae, and Myoviridae for BS1, BS2 and BS3 respectively. Bradford showed the total protein of phages were 0.23, 0.14 and 0.28 mg/ml for BS1, BS2 and BS3 respectively. SDS-PAGE showed molecular weight of protein was ~ 97, 70, 37, and 35 KDa in BS1. In BS2 was ∼120, 97, 70, 35 and 25 KDa. BS3 was ∼97, 70, 35 and 27 KDa. DNA. RAPD-PCR showed. BS1 amplified to 3 fragments with 1000, 600 and 500 bp, phage BS2 amplified to 2 fragments with 1000 and 600 bp, BS3 phage amplified to 2 fragments with 1000 and 500 bp . Five formulas were used to protect phages from UV irradiation. The best used formulas for that used with all phages were Beet-root juice and Casein formulas.

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